Microscope Illuminator

By Stefan Martin

This example script demonstrates how to switch between different LED channels and change the illuminator intensity, using the X-LCA4 lighting controller and MLR3 illuminaor for multi-channel imaging of flourescent targets using multiple fluorophores.

Hardware

This script is intended for use with the following hardware:

• X-LCA4 microscope lighting controller
• MLR3 transmitted illuminator
• MLT epi-illuminator modules

Dependencies

The script uses the following package:

• Zaber Motion Library

Configuration

Edit the following constants in the script to fit your setup before running the script:

• SERIAL_PORT: the serial port that your device is connected to. For more information on how to identify the serial port, see Find the right serial port name.

Use

• set_intensity(Axis, percent): Set the intensity by a percentage or (optionally) using a particular luminous flux in watts.
• pulse_channel(Axis, duration): Turn on the channel for a given time in milliseconds. Returns once the duration is over, but this wait can be removed if you'd prefer to use a signal from the camera.

Multi-Dimensional Acquisition

This script can be combined with other example scripts to automate complex multi-dimensional imaging processes:

• microscope_high_throughput_scanning
• microscope_filter_cube